Description
Aviva Systems Biology HLA-DRA ELISA Kit (Human) (OKCA00694) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HLA-DRA has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HLA-DRA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample HLA-DRA captured in the well